Ignore the chaos in the background. 😅 My dog came through and knocked everything over with the wiggles.

      • Doxatek@mander.xyz
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        1 year ago

        Haha good luck! I do tissue culture for a living. You definitely can do it at home but you have a lot of work cut out for you

        • Salamander@mander.xyz
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          1 year ago

          Haha, thanks! I have a few years of experience with home mycology (agar work/sterile technique) and I also keep lots of houseplants, so I’m thinking some of these skills might transfer when I do get into it. A reason why I’m interested in this is because some of the work I’ve read about fungi-root interactions was performed on fungi grown on root tissue cultures - which makes sense - but I don’t really know how one would practically achieve that. So I’m very curious about it…

          • Doxatek@mander.xyz
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            1 year ago

            Gotcha. While never having cultured fungus (on purpose) plants definitely pose unique challenges. Such as hormone application. I also think, though I don’t have extensive knowledge and fungal media composition, that the media for plants can need quite a lot more sugar which makes bacteria and fungi even more a problem.

            Really cool you’ve already done it at home though. Did you do pressure cooker method

            • Salamander@mander.xyz
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              1 year ago

              Such as hormone application.

              Oh, I haven’t worked with hormones before. That would be interesting.

              Did you do pressure cooker method

              Yeah, my pressure cookers are basically my autoclaves. And I use a still-air box (30L transparent plastic tub with holes) for pouring plates and transfers.